Select the “Show Uncompensated Parameters”. If you would always like to see the uncompensated parameters in the graph window, click on the Graph preference set in the FlowJo preferences. Therefore, when you add the data files to a FlowJo workspace, they show up with a color grid (badge) beside them to indicate they have been compensated.Īnother benefit of FCS3.0 data is that uncompensated parameters can always be viewed. If the data was compensated during acquisition, this compensation matrix is included in the data file (in FlowJo, it is referred to as the Acquisition matrix).įlowJo will apply the Acquisition matrix to any data files that contain the embedded matrix. Import to FlowJoĭata exported from the BD FACSDiva software (except FCS2.0 files) is saved in linear (no log or biexponential scaling), uncompensated format. However, the information lost with FCS2.0 data is rarely worth the minor increase in performance. FCS2.0 files will have less information than FCS3.0 or FSC3.1 data, so they may be faster. If you do not wish to use advanced compensation or biexponential transformation settings, then export your data as FCS2.0. Not all keywords modified after acquisition will be exported from Diva, but you can fix this by copying/pasting a spreadsheet of your keywords to label the data in FlowJo. Some of the older versions transposed the compensation matrix. Older BD FACSDiva versions – you may sometimes wish to export as Experiment. We highly recommend exporting as digital FCS3.0 data: 18 bit resolution (262,144 channels). Export from DivaĬurrent BD FACSDiva version 6.x can export data two ways “Export Experiment” or “Export FCS files”. Did you acquire your data with BD’s FACSDiva software? Compensate during acquisition? No problem! FlowJo can read FACSDiva data files and apply the compensation matrix you used at the time of acquisition automatically.
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